Plasmid_Backbone

Part:BBa_K1621009:Design

Designed by: Ramona Emig, Julika Neumann, Lara Stuehn   Group: iGEM15_Freiburg   (2015-08-30)


pOP - protein expression vector compatible with RFC[25]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 4984
    Illegal suffix found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4984
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 4990
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4984
    Illegal BglII site found at 4837
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4984
    Illegal suffix found at 11
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1054


Design Notes

The original sequence of pET22b+ was modified to make it compatible to iGEM standard cloning procedures. This includes the removal of recognition sites of several restriction enzymes as well as the insertion of RFC[25] as a multiple cloning site. Further details can be found on the main page.


Source

The sequence is a modified version of the common expression vector pET22b+. Five fragments that were needed for the assembly of the whole part were synthesized by Integrated DNA Technologies.

References